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TIRFM (Total Internal Reflection Fluorescence Microscopy System)
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Olympus' original high N.A. objectives make it easy to
produce an evanescent wave field. So little light is leaked that a high-contrast
image can be obtained against a dark background. * N.A. 1.65, 100X objective (APO100XOHR). Incompatible resolution and brightness (use special cover glass and immersion oil) * N.A. 1.45 objectives (PLAPON60XOTIRFM, PLAPO100XOTIRFM) UPLSAPO100XO (N.A. 1.4) (Use normal cover glass and immersion oil) After initial alignment of the single mode fiber to the laser head and TIRFM illuminator, a simple micrometer adjustment is all that is required to move the laser position between TIRFM and widefield illumination. Application Cell Surface Imaging - D. Axelrod - Cell/Substrate Contact / W. Reichert Single molecule imaging - Myosin, Actin with Cy3 labeled ATP / T. Yanagida Exocytosis (w/ High Speed Camera) - Exocytosis of single secretory granules in live cells using acridine orange / W. Almers - Using NA1.65 Objective Lens/ Dr. Terakawa Cardiac Ca2+ sparks (w/ High Speed Camera) - Using Ca-green / M. Morad |