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TIRFM (Total Internal Reflection Fluorescence Microscopy System)

Since 1997, Olympus has been a market leader in objective based Total Internal Reflection systems that allows evansecent wave illumination approximately 200nm into the specimen beyond the coverglass interface. Olympus extends that leadership role by offering three objectives for TIRFM including the world's highest NA objective, the 100X N.A. 1.65 objective.
The incredibly thin optical section created be TIRFM allows an extremely high signal to noise image to be collected. Popular applications include vesicle tracking, cellular adhesions and single molecule events.

 Olympus' original high N.A. objectives make it easy to produce an evanescent wave field. So little light is leaked that a high-contrast  image can be obtained against a dark background.
  * N.A. 1.65, 100X objective (APO100XOHR).
 Incompatible resolution and brightness (use special cover glass and immersion oil)

  * N.A. 1.45 objectives (PLAPON60XOTIRFM, PLAPO100XOTIRFM)
    UPLSAPO100XO (N.A. 1.4) (Use normal cover glass and immersion oil)

 After initial alignment of the single mode fiber to the laser head and TIRFM illuminator, a simple micrometer adjustment is all that is  required to move the laser position between TIRFM and widefield illumination.

 
Application
  Cell Surface Imaging
   - D. Axelrod
   - Cell/Substrate Contact / W. Reichert
      Single molecule imaging
   - Myosin, Actin with Cy3 labeled ATP / T. Yanagida
      Exocytosis (w/ High Speed Camera)
   - Exocytosis of single secretory granules in live cells using acridine orange / W. Almers
   - Using NA1.65 Objective Lens/ Dr. Terakawa
     Cardiac Ca2+ sparks
     (w/ High Speed Camera)
   - Using Ca-green / M. Morad